E20-06: Practical NMR Spectroscopy

Wednesday, Oct. 14 – Thursday, Oct. 15; 10:00am – 2:30pm

Dr. Damodaran Achary, University of Pittsburgh, Pittsburgh PA

In this course, you will learn the fundamentals of NMR spectroscopy that will help you better understand commonly used and advanced 1D and 2D NMR experiments, both in routine analytical work and in scientific research. Emphasis will be given on practical aspects of NMR spectroscopy. You will also learn the tips and tricks to obtain the best out of your spectrometer. Overall, this course will prepare you to engage the NMR spectrometer and run experiments with confidence.

This course is designed to deliver practical aspects and the underlying concepts in NMR spectroscopy. Either you are new to the field or you want to further your understanding of NMR spectroscopy, this is the course that will provide that training for you. It will help you gain knowledge in NMR concepts, applications, data processing, spectral analysis and interpretation. It will also prepare you for the growing needs for highly skilled NMR personnel in academia and industry. Overall this course is for anyone who wants to improve their knowledge in NMR.

  1. Introduction to NMR Spectroscopy
    a. Interactions in NMR spectroscopy and their implications on the NMR spectrum
    b. Multiplicity, spin-spin coupling and patterns
    c. Primer on NMR pulse and sequences
    d. Overview of NMR hardware
  2. 1D NMR Spectroscopy
    a. Anatomy of an FID (Free Induction Decay); signal to noise vs resolution
    b. Factors that affect the quality of the NMR data
    c. Acquisition and Processing techniques; Tips and tricks
    d. Multinuclear NMR experiments
  3. 2D NMR Spectroscopy for structural elucidation
    a. Nuts and Bolts of 2D NMR spectroscopy
    b. Homonuclear correlation: COSY, TOCSY, NOESY, ROESY
    c. Heteronuclear correlation: HSQC, HMQC, HMBC
    d. Parameters that influence the quality of 2D NMR data
  4. Advanced 1D and 2D NMR techniques
    a. Selective NMR experiments
    b. Non-Uniform Sampling (NUS); Resolution vs Speed
    c. Pure shift NMR for ultrahigh resolution 1D and 2D data and dynamic NMR spectroscopy

Dr. Damodaran Achary received his B.Sc. from the University of Madras, India and his M.Sc. in Physical Chemistry from the University of Pune, India. He received his Ph.D. in Solid State NMR Spectroscopy from the National Chemical Laboratory, India. He then moved to the US to do his post-doc at Ames Laboratory, Iowa. Later he took an NMR Spectroscopist/Facility Manager position at Miami University, Oxford, OH. In 2005, he moved to the University of Pittsburgh as the Director of the NMR Facility which he currently holds. His research interests include application of solution and solid state NMR spectroscopy to interdisciplinary areas of science. He has extensive experience training hundreds of academic and industry users and also taught courses on NMR spectroscopy for chemists. He has co-authored over 60 peer-reviewed publications applying NMR spectroscopy to a variety of fields.