{"id":3257,"date":"2020-02-22T18:02:04","date_gmt":"2020-02-22T23:02:04","guid":{"rendered":"http:\/\/easinc.org\/wordpress\/?page_id=3257"},"modified":"2022-04-11T17:58:31","modified_gmt":"2022-04-11T21:58:31","slug":"e15-advanced-hplcuhplc-part-2","status":"publish","type":"page","link":"https:\/\/eas.org\/2024\/e15-advanced-hplcuhplc-part-2\/","title":{"rendered":"E22: HPLC and UHPLC for Practicing Scientists 1 and 2: Fundamentals, Method Development, and Troubleshooting"},"content":{"rendered":"
\n

Two-Day Course<\/span>
Date to be announced; 8:30am – 5:00pm
\n<\/i><\/strong>\u00a0Dr. Michael W. Dong, MWD Consulting, Norwalk, CT<\/span><\/p>\n<\/div>\n

COURSE DESCRIPTION<\/span>
\n<\/b>This intermediate short course will provide the analytical scientist with a clearer understanding and a solid working knowledge of best practices (method development, HPLC operation, and troubleshooting) of HPLC and UHPLC (ultra-high-pressure liquid chromatography). \u00a0The focus is on pharmaceutical analysis of small molecule drugs.\u00a0 A brief survey of biopharmaceutical, food, environmental, chemical, and bioscience applications is included.<\/span><\/p>\n

HPLC &UHPLC for Practicing Scientists 1: Fundamentals\u00a0<\/em>is the first part of this two-day course.\u00a0<\/b>Part 1 and 2 can<\/span> be combined\u00a0as a two-day course;\u00a0a discount will be offered over separately registering for the two one-day courses. (click here for Part 1 course description<\/a>)<\/em><\/span><\/p>\n

WHO SHOULD ATTEND<\/span>
\n<\/b>Analysts, scientists,\u00a0researchers, and managers in pharmaceutical and other industries who want to get an updated overview of the best practices of HPLC and UHPLC in pharmaceutical analysis including HPLC method development, operation, and troubleshooting.\u00a0 A basic understanding of chemistry and HPLC with some hands-on experience is assumed.<\/span><\/p>\n

\n

TOPICS<\/span>
\n<\/b>1. HPLC Applications and an Overview of UHPLC<\/span>
\u00a0 \u00a0 \u00a0a. HPLC applications including pharmaceutical, biopharmaceuticals, food, environmental, chemical, and bioseparations
\nb. FUHPLC overview, perspectives, and benefits: UHPLC concepts, instrumentation, and benefits (fast separations, high-resolution analysis, and rapid method development)<\/span>
\u00a0 \u00a0 \u00a0c.\u00a0Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC, transition from HPLC to UHPLC\u00a0<\/span>
\n<\/span>2. HPLC Method Development and Validation
\n<\/b>\u00a0 \u00a0 \u00a0a. Tradition strategy for HPLC method development and a survey of automated tools and software (DryLab, ACD AutoChrom, and Fusion QbD) \u00a0<\/span>
\n\u00a0 \u00a0 \u00a0b.\u00a03-pronged template approach for rapid method development <\/span>
\n\u00a0 \u00a0 \u00a0c. Use of a universal generic gradient method for the assay of multiple NCEs and stability-indicating assays with simple adjustments
\nd.\u00a0A brief overview of method validation and method transfer with case studies
\n<\/span>3. HPLC Operation and Troubleshooting<\/span>
\n\u00a0 \u00a0 \u00a0a.\u00a0Mobile phase and sample preparation and best practice in HPLC operation <\/span>
\n\u00a0 \u00a0 \u00a0b.\u00a0Maintenance and troubleshooting guide with case studies <\/span>
\n\u00a0 \u00a0 \u00a0c.\u00a0Diagnosing and solving problems (pressure, baseline, peak, data performance) <\/span><\/p>\n<\/div>\n

<\/a>ABOUT THE INSTRUCTOR
\n<\/b><\/b>Dr. Michael W. Dong<\/b> is\u00a0a\u00a0principal\u00a0of MWD Consulting focusing on training and consulting on HPLC\/UHPLC, method development, and pharmaceutical analysis.\u00a0 He was formerly Senior Scientist at Genentech, Research Fellow at Purdue Pharma, and Senior Staff Scientist at Applied Biosystems\/Perkin-Elmer.\u00a0 He holds a Ph.D. in Analytical Chemistry from the City University of New York, and a certificate in Biotechnology from University California of Santa Cruz. He has 120+ publications including a bestselling book on chromatography (HPLC and UHPLC for Practicing Scientists, Wiley). He is an editorial advisory board member of LCGC North America, American Pharmaceutical Review, and Chinese American Chromatography Association.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Two-Day Course Date to be announced; 8:30am – 5:00pm \u00a0Dr. Michael W. Dong, MWD Consulting, Norwalk, CT COURSE DESCRIPTION This intermediate short course […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-3257","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages\/3257"}],"collection":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/comments?post=3257"}],"version-history":[{"count":25,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages\/3257\/revisions"}],"predecessor-version":[{"id":8380,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages\/3257\/revisions\/8380"}],"wp:attachment":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/media?parent=3257"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}