{"id":9564,"date":"2023-07-18T10:14:53","date_gmt":"2023-07-18T14:14:53","guid":{"rendered":"https:\/\/eas.org\/2024\/?page_id=9564"},"modified":"2023-07-18T10:14:53","modified_gmt":"2023-07-18T14:14:53","slug":"hplc-part-1-only","status":"publish","type":"page","link":"https:\/\/eas.org\/2024\/hplc-part-1-only\/","title":{"rendered":"E23-02 HPLC and UHPLC for Practicing Scientists Part 1 ONLY: Fundamentals"},"content":{"rendered":"\n
One–<\/em>Day Course
Sunday, Nov. 12; 8:30am – 5:00pm<\/h6>\n\n\n\n

Dr. Michael W. Dong, MWD Consulting, Norwalk, CT<\/p>\n\n\n\n

COURSE DESCRIPTION
<\/b>This intermediate course will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, applications, and best practices in method development and troubleshooting of HPLC and UHPLC (ultra-high-pressure liquid chromatography).\u00a0\u00a0The focus is on pharmaceutical analysis of small molecule drugs. This is Part 1 of a two-day course.<\/strong><\/span><\/p>\n\n\n\n

WHO SHOULD ATTEND
<\/b>Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of fundamentals and best practice of modern HPLC\/UHPLC in pharmaceutical analysis and other applications including HPLC method development, operation and troubleshooting.  A basic understanding of chemistry and HPLC with some hands-on experience is assumed.<\/span><\/p>\n\n\n\n

Day 1: HPLC 1: Fundamentals:<\/h6>\n\n\n\n
    \n
  1. Concepts
    a. Introduction: The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)
    b. Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs<\/sub>), tailing factor (Tf<\/sub>), the resolution equation, the linear solvent strength theory, and column void volume (VM<\/sub>)
    c. Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG<\/sub>), column temperature (T)), peak capacity (Pc<\/sub>)<\/li>\n\n\n\n
  2. HPLC Columns, Trends and Selection Guides
    a. Column fundamentals: Glossary, Characteristics, and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation
    b. Trends: Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, concept of \u201corthogonality,\u201d  listing of common RPC columns, column selection guide
    c. Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2 mm, and superficially porous particles (SPP)<\/li>\n\n\n\n
  3. HPLC Instrumentation and Operating Principles
    a. System, Pump, and Autosampler. Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume, pump trends, autosampler types and trends
    b. Detectors: Operating principles of UV\/Vis detection and characteristics, photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD, and CLND)
    c. MS, CDS, and Instrument Bandwidth. The mass spectrometer (MS), types and ESI interface, Data Chromatography System (CDS), peak integration strategy, instrumental bandwidth<\/li>\n<\/ol>\n\n\n\n
    \"\"<\/figure>\n\n\n\n

    <\/a>ABOUT THE INSTRUCTOR
    <\/b>Dr. Michael W. Dong<\/b>\u00a0is\u00a0a principal of MWD Consulting <\/span><\/p>\n\n\n\n

    focusing on consulting and training services on HPLC, pharmaceutical analysis, and drug quality. \u00a0He was formerly a Senior Scientist in Analytical Chemistry and Quality Control at Genentech, \u00a0Research Fellow at Purdue Pharma, and Senior Staff Scientist at Applied Biosystems\/Perkin-Elmer. He holds a Ph.D. in Analytical Chemistry from the City University of New York and a certificate in Biotechnology from the University of California Santa Cruz.\u00a0 He has 130+ publications and five books, including a bestselling book on chromatography (HPLC and UHPLC for Practicing Scientists, 2nd<\/sup> Ed., Wiley, 2019). \u00a0He is an advisory board member of LCGC magazine and the Chinese American Chromatography Association.\u00a0 He has been a columnist for \u201cPerspectives of Modern HPLC\u201d<\/em> for LCGC North America since 2013.\u00a0 Michael was born in Shanghai and raised in Hong Kong. \u00a0He is multilingual, an Eagle Scout, and a long-term Toastmaster.<\/p>\n","protected":false},"excerpt":{"rendered":"

    One–Day CourseSunday, Nov. 12; 8:30am – 5:00pm Dr. Michael W. Dong, MWD Consulting, Norwalk, CT COURSE DESCRIPTIONThis intermediate course will provide the analytical […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-9564","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages\/9564"}],"collection":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/comments?post=9564"}],"version-history":[{"count":0,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages\/9564\/revisions"}],"wp:attachment":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/media?parent=9564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}