{"id":9744,"date":"2020-02-22T11:34:03","date_gmt":"2020-02-22T16:34:03","guid":{"rendered":"http:\/\/easinc.org\/wordpress\/?p=1122"},"modified":"2020-02-22T11:34:03","modified_gmt":"2020-02-22T16:34:03","slug":"e13-essentials-of-modern-hplcuhplc-1-2","status":"publish","type":"page","link":"https:\/\/eas.org\/2024\/e13-essentials-of-modern-hplcuhplc-1-2\/","title":{"rendered":"E23-02: HPLC & UHPLC for Practicing Scientists Part 1: Fundamentals"},"content":{"rendered":"
\n

\u00a0<\/span><\/b><\/span>One-D<\/i><\/strong><\/span>ay Course
<\/span><\/strong><\/span>Sunday, Nov. 12; 8:30am – 5:00pm
\n<\/strong><\/i>\u00a0Dr. Michael W. Dong, MWD Consulting, Norwalk, CT<\/span><\/p>\n<\/div>\n

COURSE DESCRIPTION
<\/span><\/b>This intermediate 1-day course will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, and columns of HPLC and UHPLC (ultra-high-pressure liquid chromatography). \u00a0The focus is on pharmaceutical analysis of small molecule drugs.<\/span><\/p>\n

HPLC & UHPLC for Practicing Scientists Part 2: Best Practices in Method Development and Operation\/Troubleshooting<\/em>\u00a0<\/span>is the second part of this two-day course.\u00a0<\/b>Part 1 and 2 can be combined\u00a0as a two-day course;\u00a0a discount will be offered over separately registering for the two one-day courses. \u00a0(click here for Part 2 course description<\/a>)<\/em><\/span><\/p>\n

WHO SHOULD ATTEND
<\/span><\/b>Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of fundamentals of HPLC and UHPLC in pharmaceutical analysis and other applications.\u00a0 A basic understanding of chemistry and HPLC with some hands-on experience is assumed.<\/span><\/p>\n

\n

TOPICS
\n<\/b><\/span>1. Concepts<\/span>
\n\u00a0 \u00a0 \u00a0a. Introduction:\u00a0 The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)<\/span>
\n\u00a0 \u00a0 \u00a0b. Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs<\/sub>), tailing factor (Tf<\/sub>), the resolution equation, the linear solvent strength theory, and column void volume (VM<\/sub>)<\/span>
\n\u00a0 \u00a0 \u00a0c. Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG<\/sub>), column temperature (T)), peak capacity (Pc<\/sub>)<\/span>
\n 2.\u00a0HPLC Columns, Trends and Selection Guides<\/span>
\n\u00a0 \u00a0 \u00a0a. Column fundamentals: Glossary, Characteristics, and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation<\/span>
\n\u00a0 \u00a0 \u00a0b. Trends: Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, concept of \u201corthogonality,\u201d \u00a0listing of common RPC columns, column selection guide<\/span>
\n\u00a0 \u00a0 \u00a0c. Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2 mm, and superficially porous particles (SPP)<\/span>
\n 3.\u00a0HPLC instrumentation and Operating Principles<\/span>
\n\u00a0 \u00a0 \u00a0a. System, Pump, and Autosampler. Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume, pump trends, autosampler types and trends<\/span>
\n\u00a0 \u00a0 \u00a0b. Detectors: Operating principles of UV\/Vis detection and characteristics,\u00a0\u00a0 photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD, and CLND)<\/span>
\n\u00a0 \u00a0 \u00a0 c. MS, CDS, and Instrument Bandwidth. The mass spectrometer (MS), types and ESI interface, Data Chromatography System (CDS), peak integration strategy, instrumental bandwidth<\/span><\/p>\n<\/div>\n

<\/a>ABOUT THE INSTRUCTOR
<\/span><\/b>\u00a0<\/b>Dr. Michael W. Dong<\/b> <\/span>is\u00a0a principal of MWD Consulting focusing on training and consulting on HPLC\/UHPLC, method development, and pharmaceutical analysis.\u00a0 He was formerly Senior Scientist at Genentech, Research Fellow at Purdue Pharma, and Senior Staff Scientist at Applied Biosystems\/Perkin-Elmer.\u00a0 He holds a Ph.D. in Analytical Chemistry from the City University of New York, and a certificate in Biotechnology from University California of Santa Cruz. He has 120+ publications including a bestselling book on chromatography (HPLC and UHPLC for Practicing Scientists, Wiley). He is an editorial advisory board member of LCGC North America, American Pharmaceutical Review, and Chinese American Chromatography Association.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0One-Day CourseSunday, Nov. 12; 8:30am – 5:00pm \u00a0Dr. Michael W. Dong, MWD Consulting, Norwalk, CT COURSE DESCRIPTIONThis intermediate 1-day course will provide the […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-9744","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages\/9744"}],"collection":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/comments?post=9744"}],"version-history":[{"count":0,"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/pages\/9744\/revisions"}],"wp:attachment":[{"href":"https:\/\/eas.org\/2024\/wp-json\/wp\/v2\/media?parent=9744"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}