E20-07: Learning Chromatography with LC Simulator

Monday, Oct. 19 – Tuesday, Oct. 20; 10:00am – 2:30pm

Dr. Martin Gilar, Waters Corp.,  Milford, MA

The course attendees will learn about principles of chromatographic modes including reversed-phase, hydrophilic interaction, ion-exchange and size exclusion chromatography. Special emphasis will be given to reversed phase mode. Couse attendees will use MS Excel spreadsheets prepared to visualize chromatograms. Attendees will be able to change parameters such as particle size, column length, separation condition, extra column dispersion and others and instantly observe the effects in chromatographic separation.

Chromatography practitioners, lab personal, students, casual LC users who are interested in understanding of chromatographic principles. The attendees should bring laptop computers with installed MS Excel. We will perform in-silico chromatographic with provided MS excel chromatogram simulators.

  1. Principles of Chromatographic Separation, Isocratic Retention Versus Gradient.
  2. Retention, Resolution, Peak Capacity, Band Broadening, Extra Column Dispersion.
  3. Chromatographic Behavior of Small Molecules Versus Biopolymers.
  4. Approaches to Method Development and Method Transfer.
  5. SPE Versus LC.
  6. Chromatographic Artifacts of Large Volume Injection, Incompatible Solvents, etc.
  7. Independent In-Silico LC Experiments.

Questions and answers. Correct answers will be rewarded with silica (quartz crystals – variety “Herkimer diamonds”)

Dr. Martin Gilar received Ph.D. in analytical chemistry from Institute of Chemical Technology in Prague (1996). He spent postdoc years at Hybridon Inc. (1996-1998) and Northeastern University in Boston (1998) developing separation methods for antisense oligonucleotides and fraction collector for DNA molecules. Since 1998 he works at Waters Corp. in Milford, Massachusetts, participating in column, sample preparation and instrument research. Dr. Martin Gilar is a Scientific Fellow in Separation Science Research group. He has over 25 years of experience in the separation sciences, including chromatography, electrophoresis, and mass spectrometry. His research interest is the analysis of biopolymers, and 2D LC. He has published over 55 peer reviewed papers.