E16-26: Sample Preparation: The Chemistry Behind the Techniques

One-Day Course 
Tuesday, November 15, 8:30am – 5:00pm

 Dr. Douglas E. Raynie, South Dakota State University, SD
Dr. Merlin K. L. Bicking, ACCTA, Inc., Woodbury, MN

Come prepared to learn that sample preparation is more than just a few “low tech” procedures.  Learn about the chemical principles behind the techniques, and how an understanding of these principles will produce better results in your laboratory.  This course will include a survey of many traditional procedures, including information on recent advances in these techniques.  Several new sample preparation technologies will also be introduced.

This is not a “recipe” course limited to a particular sample type or application.  This course offers a comprehensive treatment of sample preparation as an important part of every analytical method.  You will learn more than just a few manipulations; you will come away with a complete understanding of what sample preparation is and how you can use it!  This has been a popular course at EAS for many years, and has been updated to provide you with a good understanding of modern sample preparation.

Analytical chemists from all areas, especially those who want to learn more about sample preparation techniques, including preparation laboratory staff, analysts, and supervisors, will benefit from this course.

1. Perspectives on the Importance of Sample Preparation
2. General Principles Used in Sample Preparation Procedures
     * Physical changes, LeChatelier’s Principle
     * Effects of temperature, time, ionic strength, and pH
     * Like-dissolves-like
     * Two-phase partitioning equilibria
3. Traditional Laboratory Procedures
     * Filtration
     * Solvent evaporation
     * Solvent exchange
4. Traditional Laboratory Techniques
     * Derivatization
     * Liquid-liquid extraction (techniques, variations, and recent advances)
     * Liquid-solid extraction (traditional technologies and recent advances)
     * Solid phase extraction (SPE)
     * Membrane disk extractions
5. “New” Sample Preparation Technologies
     * GC sample preparation (headspace, thermal desorption)
     * Supercritical fluid extraction (SFE)
     * Solid phase micro extraction (SPME)
     * Accelerated solvent extraction (ASE)
6. Other new ideas (SBSE and others)

Dr. Douglas E. Raynie (Course Director) is a Research Assistant Professor in the Department of Chemistry and Biochemistry at South Dakota State University.  Prior to joining SDSU, he was employed for eleven years as a Senior Scientist at Procter and Gamble’s Corporate Research Division.  He earned his Ph.D. at Brigham Young University under the direction of Dr. Milton L. Lee.  His undergraduate degree is from Augustana (South Dakota) College, with majors in chemistry and biology.  Analytical separations research in Dr. Raynie’s laboratory includes high-resolution chromatography (high-temperature LC and SFC), chromatographic sample preparation (ASE, SFE, SPME, and SPE), chromatography theory, green analytical chemistry, and problem-based learning in analytical chemistry.

Dr. Merlin K. L. Bicking is President, ACCTA, Inc.  He has extensive analytical chemistry experience in academia, contract research, independent testing laboratories, consulting, and technical training. His professional history includes development of two EPA methods, as well as numerous methods in other regulated and non-regulated industries. His publications and presentations cover a wide range of topics, including liquid chromatography theory, derivatization, method optimization, and the use of experimental design strategies in analytical chemistry. He also develops and presents technical training seminars for analytical laboratory staff, and has been teaching EAS short courses for nearly twenty years.