E23-03: Sample Preparation: The Chemistry Behind the Techniques

One-Day Course 
Sunday, Nov. 12; 8:30am – 5:00pm

Dr. Douglas Raynie, South Dakota State University, Brookings, SD

This short course is designed to provide participants with an in-depth understanding of the role of sampling and sample preparation in analytical chemistry, especially as related to chromatography. Upon successful completion of the course, the participant will understand sampling considerations and approaches, and an overview of sample preparation strategies. Throughout the course practical and theoretical aspects of the outlined topics and application case studies will be presented.

Laboratory technicians and managers, especially those interesting in learning when to apply different sample preparation techniques and exploring new sample preparation methodology.

1. Introduction
a. Why sample preparation
b. Sample preparation trends
2. Sampling and Sample Considerations
a. Types of samples
b. Sampling statistics
c. Reduction of sample size and particle size
3. General Extraction Considerations
  a. Physical parameters
b. Classification of extraction methods
c. Extraction kinetics
4. Extraction from Liquid Samples
a. Distribution ratio
b. Liquid-liquid extraction
c. Solvent selection, including green solvents
  d. Microscale methods
  e. Solid-phase extraction
g. Solid-phase microextraction
h. Other sorbent- and membrane-based methods
5. Extraction from Solid Samples
a. Soxhlet extraction
b. Accelerated solvent extraction
c. Supercritical fluid extraction
d. Microwave-assisted extraction
  e. Ultrasound extraction
6. Tissue Extractions
7. Extraction of Volatile Analytes
a. Static and dynamic headspace sampling
b. Purge and trap
8. Post-Extraction Sample Handling
a. Solvent evaporation
  b. Sample derivatization

Dr. Douglas Raynie
is Professor Emeritus in the Department of Chemistry & Biochemistry at South Dakota State University. Prior to joining SDSU, he was employed for eleven years as a Senior Scientist at Procter and Gamble’s Corporate Research Division. He earned his Ph.D. at Brigham Young University under the direction of Dr. Milton L. Lee. Dr. Raynie’s research interests include high-resolution chromatography (including high-temperature LC and SFC), chromatographic sample preparation (including ASE, SFE, SPME, and SPE), chromatography theory, green chemistry, and problem-based learning in analytical chemistry. He is recipient of the L.S. Palmer Award from the Minnesota Chromatography Forum and is author of the Sample Preparation Perspectives column for LC/GC.