E19-18: Interpretation of Mass Spectra with Practical Solutions to Problems

One-Day Course
Monday, Nov. 18; 8:30am – 5:00pm

Dr. Mike S. Lee, Milestone Development, Newtown, PA

This introductory course covers the theory and practical interpretation of mass spectra of organic compounds and proteins/peptides through the use of practical examples. The principles of interpretation are to be illustrated by various mass spectral data from EI, CI, ESI, APCI, and MALDI-MS. This course emphasizes problem-solving skills with examples encountered in industrial and academic research, including structural characterization of trace level impurities and degradation products, analysis of natural products, identification of drug metabolites and structural determination of proteins/peptides. This course provides information on methods and technologies, enabling the scientist to address the challenges experienced in today’s modern laboratory setting.

This course is ideal for a wide range of practicing mass spectrometry scientists that range from new users, experienced professionals, chromatographers, analytical chemists, protein chemists and laboratory managers. The purpose is to provide a foundation of knowledge and practical skills leading the generation of reliable information for research, development, and manufacturing.

1. Structure Analysis Matrix
2. Definitions, Tools, Industry Trends
3. Chromatography and Mass Spectrometry Overview
4. Chemistry, Method Development, Ionization
5. Template Strategy for Structure Identification
6. Build the puzzle!
7. Simple Rearrangements
8. Nitrogen Rule, Rules!
9. Case Studies
10. Predictive Models and Tools

Dr. Mike S. Lee is President of Milestone Development Services. He actively participates in the growth of ne w technologies and their integration into drug development. Dr. Lee has extensive experience with pharmaceutical analysis and drug development. He has pioneered the application of LC/MS in different phases of drug development for research in biomolecules, natural products, drug metabolites, impurities, and degradants. Prior to founding Milestone Development Services, Dr. Lee was with Bristol-Myers Squibb from 1987-1998. As Director of Analytical Research and Development, Dr. Lee was responsible for departments in several research facilities providing MS, NMR, IR, HPLC, CE, and physical chemistry support. He led interdisciplinary teams responsible for rapid analysis of discovery leads and preclinical drug candidates that contributed to the Food and Drug Administration approach of Buspar®, and Serzone®, and the accelerated development and approval of TAXOL®. Dr. Lee has published over 40 research papers and book chapters about the analysis of drugs and related compounds. The book “Mass Spectrometry Handbook” that features an extensive library of mass spectrometry methods from leading researchers and edited by Dr. Lee was recently published by J. Wiley & Sons. Dr. Lee received his B.S. in Chemistry from the University of Maryland and his M.S. and Ph.D. in Analytical Chemistry from the University of Florida under the direction of Professor Richard A. Yost.