E23-07: High-Performance Thin-Layer Chromatography an Alternative Approach to Quality: Standardization, Quantification and Automation

One-Day Course
Sunday, November 12; 8:30am – 5:00pm

James Kababick, Flora Research Laboratories, Grand Pass, OR
Dr. Wilmer Perera, CAMAG Scientific, Wilmington, NC

High-performance thin-layer chromatography (HPTLC) has become widely accepted as reliable analytical method for identification of botanicals in a cGxP environment. It is also an established quantitative tool in various fields of application. The unique features of HPTLC fingerprints and the new concept of comprehensive HPTLC fingerprinting offer complementary and orthogonal approaches to describing the quality of complex samples. Now, multiple fingerprints from complementary chromatographic systems and multiple detection modes based on fully automatic analyses are realty and could transfer HPTLC into a new technique of instrumental analysis applied to routine quality control.

This course will start with a brief discussion of concepts, parameters and unique features of standard HPTLC. Then, it will explore new possibilities that arise from rigorous standardization combined with full automation and modern approaches to intelligent data evaluation. Interactive case studies examine and highlight the advantages of the technique. Practical sessions emphasize strategies for developing, fine-tuning, and validating qualitative and quantitative HPTLC methods. An open forum will provide opportunities to discuss new concepts and arising possibilities.

Researchers, Scientists, Academicians, QA/QC/R&D Personnel, Lab Managers and Technicians
Anyone wanting to learn about the extraordinary capabilities of HPTLC.
Anyone wanting to learn about how to get the most of an existing HPTLC system.
Anyone who wants to start thinking out of the box of liquid chromatography

1. Concepts, Parameters, and Special Features of HPTLC
a. What is so special about HP-TLC?
b. Rigorous standardization and flexible hyphenation – the two strong elements of HPTLC
c. Automatic HPTLC – the new basis for new analytical approaches
2. Practical Session: Generating Reliable HPTLC Data
3. Comprehensive HPTLC Fingerprinting
a. The Universal HPTLC mix in system suitability testing
b. Comprehensive HPTLC Fingerprinting: a different look at quality
c. Complementary HPTLC fingerprints
4. Case Studies: HPTLC in Practice
5. Quantitative Analysis by HPTLC
a. Principles of scanning densitometry
b. Assays and limit tests
c. Economic considerations
6. Practical Session: Development and Validation of HPTLC Methods
7. The Future of HPTLC
a. Specialized vs. universal screening methods
b. Complementary mobile phases for routine quality control
c. “New” definitions of quality
8. Open forum: questions and answers about the future of HPTLC


James Kababick is the founder and Director of Flora Research Laboratories, LLC (FRL) which specializes in the research and analysis of botanicals, dietary supplements and related compounds.  He is also the interim President of the North American Chapter of the HPTLC Association.  For many years he served as an adjunct faculty at Bastyr University where he taught botanical drug identification by microscopy and thin layer chromatography.  He serves on multiple expert committees for AOAC, USP, NIH, AHPA, and others.  James is the pioneer of the field called “Phytoforensic Science.”  Phytoforensic Science involves utilizing numerous technologies from microscopy to mass spectrometry to detect adulteration and contamination in the global food supply chain with a special focus on dietary supplements.  In 2010 James was named “Fellow of AOAC.”  He currently serves on the 2020-2025 USP Botanical Dietary Supplements & Herbal Medicines Expert Committee, the USP Joint Standard Settings Subcommittee, the Subcommittee on Modern Analytical Methods, the USP Dietary Protein Expert Committee, and the Saw Palmetto Modernization working group.  

Wilmer Perera received his bachelor’s degree in chemistry and master’s degree in organic chemistry from the University of Havana, Cuba. He also completed his doctorate in Biochemistry at the University of Liege, Belgium in 2012. He has published 40 papers in peer-reviewed journals and has performed multiple presentations in national and international meetings. He is currently the laboratory manager at CAMAG Scientific, Inc, and oversees HPTLC analyses and projects, as well as training and courses to customers in the United States of America and Canada. Dr. Perera is also the General Secretary of the North America Chapter of the International Association for the Advancement and collaborates on the HPTLC Atlas for identification of herbal drugs. He is also a USP expert advisor and participates actively in USP meetings.