E23-27: Introduction to LC-MS Metabolomics

One-Day Course:
Wednesday, Nov. 15; 8:30am – 5:00pm
Dajana Vuckovic, Concordia University, Montréal

This course will provide an introduction to untargeted (global) metabolomics and lipidomics using liquid chromatography – mass spectrometry (LC-MS). The course will cover practical and fundamental aspects of experimental design, sample preparation, LC-MS analysis, quality control, data processing, statistical interpretation including multivariate statistical analysis and metabolite identification strategies in the context of untargeted metabolomics. The most effective strategies to enhance metabolite coverage and example quality control practices for untargeted metabolomics will be discussed in detail.

This introductory course is intended for professionals or students in analytical chemistry, mass spectrometry and/or life sciences who are interested in learning the basics of metabolomics and lipidomics using liquid chromatography – mass spectrometry. The participants are expected to have basic knowledge of LC-MS and biological sample preparation.

  1. Introduction to Metabolomics and Definitions; Compare and Contrast Key Differences Between Targeted and Untargeted Analyses.
  2. Experimental Design Considerations for Successful Metabolomics and Lipidomics Studies.
  3. Analytical Workflows for Untargeted LC-MS Metabolomics.
    a. Describing the independent (X block) variables.
    b. Describing the dependent (Y block) variables.
    c. Analyzing residual plots.
    d. Introduction to chemometric methods.
  4. LC-MS Metabolomics Versus Lipidomics.
  5. Quality Control Practices and Example Case Studies in Metabolomics and Lipidomics.
  6. Wrap up and Q&A.

Dr. Dajana Vuckovic
is an Associate Professor in the Department of Chemistry and Biochemistry and the Director of the Centre for Biological Applications of Mass Spectrometry at Concordia University, Montreal, Canada. With more than 15 years of expertise in metabolomics, her research program focuses on the development of new analytical workflows in untargeted metabolomics in order to improve metabolite coverage and measurement of unstable metabolites such as inflammatory mediators. To date, she has authored 49 publications and 11 book chapters and delivered 47 invited talks. She is an Editorial Board member of Bioanalysis and Analytical and Bioanalytical Chemistry. She is co-chair of Metabolomics 2023, this year’s edition of the annual conference of Metabolomics Society. She also serves as the Secretary for Metabolomics Association of North America and as the co-chair of Best Practices Working Group of the Metabolomics Quality Assurance & Quality Control Consortium (mQACC).